cleancap ® reagent ag (3′ ome) n-7413 Search Results


cleancap reagent ag (3’ ome)  (TriLink)
90
TriLink cleancap reagent ag (3’ ome)
Cleancap Reagent Ag (3’ Ome), supplied by TriLink, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
cleancap reagent ag (3’ ome) - by Bioz Stars, 2026-11
90/100 stars
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trinucleotide cap1 analog (m7(3’omeg)(5’)ppp(5’)(2’omea)pg, n-7413  (TriLink)
90
TriLink trinucleotide cap1 analog (m7(3’omeg)(5’)ppp(5’)(2’omea)pg, n-7413
Trinucleotide Cap1 Analog (M7(3’omeg)(5’)Ppp(5’)(2’omea)pg, N 7413, supplied by TriLink, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cleancap+%C2%AE+reagent+ag+(3%E2%80%B2+ome)+n-7413/cleancap/pmc11349762-249-16-17
Average 90 stars, based on 1 article reviews
trinucleotide cap1 analog (m7(3’omeg)(5’)ppp(5’)(2’omea)pg, n-7413 - by Bioz Stars, 2026-11
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n 1-methylpseudouridine-5′-triphosphate, mψ  (TriLink)
90
TriLink n 1-methylpseudouridine-5′-triphosphate, mψ
N 1 Methylpseudouridine 5′ Triphosphate, Mψ, supplied by TriLink, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cleancap+%C2%AE+reagent+ag+(3%E2%80%B2+ome)+n-7413/m1%CF%88+5++triphosphate/pmc12219281-348-12-13
Average 90 stars, based on 1 article reviews
n 1-methylpseudouridine-5′-triphosphate, mψ - by Bioz Stars, 2026-11
90/100 stars
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cleancap413  (TriLink)
90
TriLink cleancap413
Cleancap413, supplied by TriLink, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cleancap+%C2%AE+reagent+ag+(3%E2%80%B2+ome)+n-7413/cleancap413/us11779659-1523-8-12
Average 90 stars, based on 1 article reviews
cleancap413 - by Bioz Stars, 2026-11
90/100 stars
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arca-g  (TriLink)
90
TriLink arca-g
Arca G, supplied by TriLink, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cleancap+%C2%AE+reagent+ag+(3%E2%80%B2+ome)+n-7413/arca/pmc08879150-64-0-3
Average 90 stars, based on 1 article reviews
arca-g - by Bioz Stars, 2026-11
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co-transcriptional capping reagent m7(3'omeg)(5')ppp(5')(2'omea)pg cap analog  (TriLink)
90
TriLink co-transcriptional capping reagent m7(3'omeg)(5')ppp(5')(2'omea)pg cap analog
Co Transcriptional Capping Reagent M7(3'omeg)(5')Ppp(5')(2'omea)pg Cap Analog, supplied by TriLink, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cleancap+%C2%AE+reagent+ag+(3%E2%80%B2+ome)+n-7413/cleancap++ag++m7g+5++ppp+5+++2+omea+pg/pmc11298514__41467_2024_51044_MOESM2_ESM-176-6-16
Average 90 stars, based on 1 article reviews
co-transcriptional capping reagent m7(3'omeg)(5')ppp(5')(2'omea)pg cap analog - by Bioz Stars, 2026-11
90/100 stars
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epo mrna (cc1  (TriLink)
90
TriLink epo mrna (cc1
LC–MS analysis of ribozyme-mediated cleavage products for quantification and characterization of capped products from Rz1 cleaved and silica-column purified enzymatically capped and 2′-O-methylated (E1), human alpha globin (hAg), m1Ψ-containing erythropoietin <t>(EPO)</t> <t>mRNA.</t> The enzymatical capping procedure provides 7MeGpppA(OMe)GGCGAACU*AGU*AU*U*-CU*U*CU*GGU*Cp (MW = 8334) and 7MeGpppAGGCGAACU*AGU*AU*U*CU*U*CU*GGU*Cp (MW = 8319) in a 7:2 ratio, resulting from an incomplete 2′O-methyl transfer. ( A ) UPLC and ( B ) MS profiles. Rz: Ribozyme; Cap: Capped product; Cap m : Capped methylated product; Cap u : Capped unmethylated product; Cap +G : Minor product; AU: Arbitrary units.
Epo Mrna (Cc1, supplied by TriLink, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cleancap+%C2%AE+reagent+ag+(3%E2%80%B2+ome)+n-7413/epo+mrna/pmc08879150-178-14-12
Average 90 stars, based on 1 article reviews
epo mrna (cc1 - by Bioz Stars, 2026-11
90/100 stars
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5-methylcytidine-5’-triphosphate  (TriLink)
90
TriLink 5-methylcytidine-5’-triphosphate
LC–MS analysis of ribozyme-mediated cleavage products for quantification and characterization of capped products from Rz1 cleaved and silica-column purified enzymatically capped and 2′-O-methylated (E1), human alpha globin (hAg), m1Ψ-containing erythropoietin <t>(EPO)</t> <t>mRNA.</t> The enzymatical capping procedure provides 7MeGpppA(OMe)GGCGAACU*AGU*AU*U*-CU*U*CU*GGU*Cp (MW = 8334) and 7MeGpppAGGCGAACU*AGU*AU*U*CU*U*CU*GGU*Cp (MW = 8319) in a 7:2 ratio, resulting from an incomplete 2′O-methyl transfer. ( A ) UPLC and ( B ) MS profiles. Rz: Ribozyme; Cap: Capped product; Cap m : Capped methylated product; Cap u : Capped unmethylated product; Cap +G : Minor product; AU: Arbitrary units.
5 Methylcytidine 5’ Triphosphate, supplied by TriLink, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cleancap+%C2%AE+reagent+ag+(3%E2%80%B2+ome)+n-7413/5+methylcytidine+triphosphate/pmc11298514-176-27-41
Average 90 stars, based on 1 article reviews
5-methylcytidine-5’-triphosphate - by Bioz Stars, 2026-11
90/100 stars
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ecorv  (New England Biolabs)
98
New England Biolabs ecorv
LC–MS analysis of ribozyme-mediated cleavage products for quantification and characterization of capped products from Rz1 cleaved and silica-column purified enzymatically capped and 2′-O-methylated (E1), human alpha globin (hAg), m1Ψ-containing erythropoietin <t>(EPO)</t> <t>mRNA.</t> The enzymatical capping procedure provides 7MeGpppA(OMe)GGCGAACU*AGU*AU*U*-CU*U*CU*GGU*Cp (MW = 8334) and 7MeGpppAGGCGAACU*AGU*AU*U*CU*U*CU*GGU*Cp (MW = 8319) in a 7:2 ratio, resulting from an incomplete 2′O-methyl transfer. ( A ) UPLC and ( B ) MS profiles. Rz: Ribozyme; Cap: Capped product; Cap m : Capped methylated product; Cap u : Capped unmethylated product; Cap +G : Minor product; AU: Arbitrary units.
Ecorv, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cleancap+%C2%AE+reagent+ag+(3%E2%80%B2+ome)+n-7413/EcoRV/custom%40r0195%4036550570
Average 98 stars, based on 1 article reviews
ecorv - by Bioz Stars, 2026-11
98/100 stars
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hiscribe t7 quick high yield rna synthesis kit  (New England Biolabs)
99
New England Biolabs hiscribe t7 quick high yield rna synthesis kit
LC–MS analysis of ribozyme-mediated cleavage products for quantification and characterization of capped products from Rz1 cleaved and silica-column purified enzymatically capped and 2′-O-methylated (E1), human alpha globin (hAg), m1Ψ-containing erythropoietin <t>(EPO)</t> <t>mRNA.</t> The enzymatical capping procedure provides 7MeGpppA(OMe)GGCGAACU*AGU*AU*U*-CU*U*CU*GGU*Cp (MW = 8334) and 7MeGpppAGGCGAACU*AGU*AU*U*CU*U*CU*GGU*Cp (MW = 8319) in a 7:2 ratio, resulting from an incomplete 2′O-methyl transfer. ( A ) UPLC and ( B ) MS profiles. Rz: Ribozyme; Cap: Capped product; Cap m : Capped methylated product; Cap u : Capped unmethylated product; Cap +G : Minor product; AU: Arbitrary units.
Hiscribe T7 Quick High Yield Rna Synthesis Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cleancap+%C2%AE+reagent+ag+(3%E2%80%B2+ome)+n-7413/HiScribe+T7+Quick+High+Yield+RNA+Synthesis+Kit/custom%40e2050%409774083
Average 99 stars, based on 1 article reviews
hiscribe t7 quick high yield rna synthesis kit - by Bioz Stars, 2026-11
99/100 stars
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rnasin plus ribonuclease inhibitor  (Promega)
90
Promega rnasin plus ribonuclease inhibitor
LC–MS analysis of ribozyme-mediated cleavage products for quantification and characterization of capped products from Rz1 cleaved and silica-column purified enzymatically capped and 2′-O-methylated (E1), human alpha globin (hAg), m1Ψ-containing erythropoietin <t>(EPO)</t> <t>mRNA.</t> The enzymatical capping procedure provides 7MeGpppA(OMe)GGCGAACU*AGU*AU*U*-CU*U*CU*GGU*Cp (MW = 8334) and 7MeGpppAGGCGAACU*AGU*AU*U*CU*U*CU*GGU*Cp (MW = 8319) in a 7:2 ratio, resulting from an incomplete 2′O-methyl transfer. ( A ) UPLC and ( B ) MS profiles. Rz: Ribozyme; Cap: Capped product; Cap m : Capped methylated product; Cap u : Capped unmethylated product; Cap +G : Minor product; AU: Arbitrary units.
Rnasin Plus Ribonuclease Inhibitor, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cleancap+%C2%AE+reagent+ag+(3%E2%80%B2+ome)+n-7413/rnase+inhibitor/pmc12130199-359-50-56
Average 90 stars, based on 1 article reviews
rnasin plus ribonuclease inhibitor - by Bioz Stars, 2026-11
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rneasy minelute cleanup kit  (Qiagen)
99
Qiagen rneasy minelute cleanup kit
LC–MS analysis of ribozyme-mediated cleavage products for quantification and characterization of capped products from Rz1 cleaved and silica-column purified enzymatically capped and 2′-O-methylated (E1), human alpha globin (hAg), m1Ψ-containing erythropoietin <t>(EPO)</t> <t>mRNA.</t> The enzymatical capping procedure provides 7MeGpppA(OMe)GGCGAACU*AGU*AU*U*-CU*U*CU*GGU*Cp (MW = 8334) and 7MeGpppAGGCGAACU*AGU*AU*U*CU*U*CU*GGU*Cp (MW = 8319) in a 7:2 ratio, resulting from an incomplete 2′O-methyl transfer. ( A ) UPLC and ( B ) MS profiles. Rz: Ribozyme; Cap: Capped product; Cap m : Capped methylated product; Cap u : Capped unmethylated product; Cap +G : Minor product; AU: Arbitrary units.
Rneasy Minelute Cleanup Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cleancap+%C2%AE+reagent+ag+(3%E2%80%B2+ome)+n-7413/RNeasy+MinElute+Cleanup+Kit/pmc13144369-68-65-69
Average 99 stars, based on 1 article reviews
rneasy minelute cleanup kit - by Bioz Stars, 2026-11
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Image Search Results


LC–MS analysis of ribozyme-mediated cleavage products for quantification and characterization of capped products from Rz1 cleaved and silica-column purified enzymatically capped and 2′-O-methylated (E1), human alpha globin (hAg), m1Ψ-containing erythropoietin (EPO) mRNA. The enzymatical capping procedure provides 7MeGpppA(OMe)GGCGAACU*AGU*AU*U*-CU*U*CU*GGU*Cp (MW = 8334) and 7MeGpppAGGCGAACU*AGU*AU*U*CU*U*CU*GGU*Cp (MW = 8319) in a 7:2 ratio, resulting from an incomplete 2′O-methyl transfer. ( A ) UPLC and ( B ) MS profiles. Rz: Ribozyme; Cap: Capped product; Cap m : Capped methylated product; Cap u : Capped unmethylated product; Cap +G : Minor product; AU: Arbitrary units.

Journal: Pharmaceutics

Article Title: Ribozyme Assays to Quantify the Capping Efficiency of In Vitro-Transcribed mRNA

doi: 10.3390/pharmaceutics14020328

Figure Lengend Snippet: LC–MS analysis of ribozyme-mediated cleavage products for quantification and characterization of capped products from Rz1 cleaved and silica-column purified enzymatically capped and 2′-O-methylated (E1), human alpha globin (hAg), m1Ψ-containing erythropoietin (EPO) mRNA. The enzymatical capping procedure provides 7MeGpppA(OMe)GGCGAACU*AGU*AU*U*-CU*U*CU*GGU*Cp (MW = 8334) and 7MeGpppAGGCGAACU*AGU*AU*U*CU*U*CU*GGU*Cp (MW = 8319) in a 7:2 ratio, resulting from an incomplete 2′O-methyl transfer. ( A ) UPLC and ( B ) MS profiles. Rz: Ribozyme; Cap: Capped product; Cap m : Capped methylated product; Cap u : Capped unmethylated product; Cap +G : Minor product; AU: Arbitrary units.

Article Snippet: UPLC and MS profiles of CleanCap ® Reagent AG (3′ OMe), N-7413 TriLink (CC1) EPO mRNA ( ) showed >99% capping, which is also in agreement with >90% capping efficiency obtained by screening of >100 CC1-capped IVT mRNAs using 21% PAGE, 8 M urea ( and data not shown).

Techniques: Liquid Chromatography with Mass Spectroscopy, Purification, Methylation

LC–MS analysis of ribozyme-mediated cleavage products for quantification and characterization of capped products from Rz1 cleaved and silica-column purified CleanCap ® Reagent AG (3′ OMe) = cap1 (CC1), human alpha globin (hAg), m1Ψ-containing erythropoietin (EPO) mRNA. Expected capped product (MW = 8347) is detected in >99%. ( A ) UPLC and ( B ) MS profiles. Rz: Ribozyme; Cap: Capped product; AU: Arbitrary units.

Journal: Pharmaceutics

Article Title: Ribozyme Assays to Quantify the Capping Efficiency of In Vitro-Transcribed mRNA

doi: 10.3390/pharmaceutics14020328

Figure Lengend Snippet: LC–MS analysis of ribozyme-mediated cleavage products for quantification and characterization of capped products from Rz1 cleaved and silica-column purified CleanCap ® Reagent AG (3′ OMe) = cap1 (CC1), human alpha globin (hAg), m1Ψ-containing erythropoietin (EPO) mRNA. Expected capped product (MW = 8347) is detected in >99%. ( A ) UPLC and ( B ) MS profiles. Rz: Ribozyme; Cap: Capped product; AU: Arbitrary units.

Article Snippet: UPLC and MS profiles of CleanCap ® Reagent AG (3′ OMe), N-7413 TriLink (CC1) EPO mRNA ( ) showed >99% capping, which is also in agreement with >90% capping efficiency obtained by screening of >100 CC1-capped IVT mRNAs using 21% PAGE, 8 M urea ( and data not shown).

Techniques: Liquid Chromatography with Mass Spectroscopy, Purification